FAQs

Send the antigen at the conditions that you feel that the antigen will remain most stable. As a rule, most proteins should be shipped on Dry Ice. Peptides and conjugated peptides in solution can be sent on ice packs. Gel slices should also be sent on ice packs. Most lyophilized antigens are stable at room temperature and can be sent without any ice at all. Cells are usually sent with ice packs. Antigen bound to nitrocellulose membranes can be sent at room temperature.

Generally, three pounds of dry ice is plenty. Use a styrofoam container with walls at least 1 inch thick. Even if the package is delayed one day, three pounds is enough for the extra day of transit.

We suggest putting the gel slice into a conical or eppendorf tube with a few drops of distilled water. The idea is to keep the gel moist in transit and in storage. Estimate how much gel there is in the tube. Send using a styrofoam box using a few ice packs to keep it cool. Please do not emulsify the gel, allow us to do it. Also, do not aliquot the gel as it is easier for us to work with it in one unit. Please do not freeze gels - sometimes they are difficult to work with after freezing.

We suggest sending all perishable antigens overnight. We suggest using either FedEx or UPS as they come to our facility daily. We generally receive all packages by 4 PM Monday through Friday; our area does not have AM delivery. Please avoid using the USPS (United States Postal Service) for antigen shipments.

Please ship to us on Monday, Tuesday, or Wednesday, so that we can expect to receive on Tuesday, Wednesday, or Thursday. Please do not send perishable antigens on Thursdays. Even the most reliable carriers can have delays, so if the package that you send on Thursday is delayed one day, it will not come here until Monday.

Shipping address is:

Pocono Rabbit Farm & Lab

306 Dutch Hill Road

Canadensis, PA 18325

No! All the overnight carriers will not deliver to our town on Saturdays.

We can accommodate and ship worldwide with the USDA Health Certificates.

All 28 Day Mighty Quick Protocols include setup, prebleed, three immunizations (including immune stimulators), one small immune bleed, final bleed, and maintenance. This protocol can be extended if additional sera is required. Price is per animal, unless noted, and the estimated volume of sera is 70mL sera.

Rabbit, Chicken, Guinea Pig, Goat,  Sheep, Rats , and Mice

Starting as low as $555, please inquire for pricing

All 49 Day Mighty Quick Protocols include setup, prebleed, four immunizations (including immune stimulators), four large immune bleeds, final bleed, and maintenance. This protocol can be extended if additional sera is required. Price is per animal, unless noted, and the estimated volume of sera is 110mL.

Rabbit, Chicken, Guinea Pig, Goat, Sheep, Rats, and Mice

Starting as low as $865, please inquire for pricing

70 Day Antibody Production Package includes setup, prebleed, four immunizations, one small immune bleed, one large immune bleed, and maintenance. This protocol can be extended if additional sera is required. This protocol is based on the PRF&L Traditional 70 Protocol offered in the past.Rabbit Intradermal Chicken Intramuscular Guinea Pig Intramuscular Rat or Mouse Intraperitoneal Goat or Sheep Intradermal

Two animals per antigen Starting as low as $765 please inquire for pricing

Single animal per antigen starting as low as $440 please inquire for pricing

84 Day Fusion Protein Protocol, a favorite for many years at Pocono Rabbit Farm & Laboratory. This protocol uses an IV injection at day 56 as a way to improve the antibody. This package includes setup, prebleed, six immunizations, two large immune bleeds, and maintenance. This protocol can be extended if additional sera is required. Approx. volume of sera provided per animal 65 to 90ml

Rabbit, Chicken, Guinea Pig, Rat, Goat ,Sheep, Mice

2 animals per antigen Starting as low as $1,095 please inquire for pricing

Single animal per antigen starting as low as $635 please inquire for pricing

Approx. volume of sera provided per animal 65 to 90ml

91 Day Antibody Production Package includes setup, prebleed, five immunizations, one small immune bleed, four large immune bleeds, and maintenance. All immunizations are subcutaneous for this package. This protocol can be extended if additional sera is required. Approx. volume of sera provided per animal 100 to 135ml

Rabbit, Chicken, Guinea Pig, Rat, Goat, Sheep, Mice

Two Animals per Antigen starting as loas as $895 inquire for pricing

Single animal per antigen starting as low as $610 inquire for pricing

Rabbit, Guinea Pig, Chicken, Goa, Sheep, Rat, Mice

Exsanguination fee very for each species please inquire for pricing.

Price includes the bleed and two weeks maintenance while you evaluate the bleeds.

Price Schedule for Monoclonal Antibody Production

Including Ascites Production

effective 09/01/2022

Monoclonal Antibody Production by In-Vivo Methods (Ascites Production) - inquire

Price per cell line, includes cell line preparation (when provided with Mycoplasma free live cell line) for immunization into 5 Balb/c Mice ( or another hybridoma specific strain maybe additional), all necessary procedures and husbandry for a typical production run which is based on removing ascites fluid a maximum of 3 times per mouse followed by euthanasia and disposal. Yields differ greatly between cell lines but if the line is a reasonable producer, 3 to 10 ml per mouse can be anticipated.

Each additional mouse added to production run by passing on the line from mouse to mouse - inquire

Protein G Purification of Ascites Fluid

approximate yield 0.5 to 2mg or > /ml of neat ascites fluid.

as low as $350

25million cells per vial per line, including storage for the first year in liquid nitrogen - inquire

Each additional year of storage - inquire

Mycoplasma Testing

First line - inqiure

Each additional lines to be tested at the same time - inquire

Isotyping

First line / Antibody - inquire

Each additional line to be tested at the same time - inquire

Quantitation of Mouse IgG in hybridoma supernatant or ascites

First Sample - inquire

Each additional sample at the same time - inquire

Conjugation to Biotin or other targets (some targets may be more)

Conjugation of up to 10mg of antibody to biotin or other target - starting at $595

Conjugation of same antigen to an additional carrier - starting at $395

Antibody Titration by ELISA

TITRATION ASSAY IN 96 WELL MICROPLATE

ASSAY PERFORMED IN DUPLICATE

10 - 20ug antigen needed per plate for coating

Protein concentration must be provided.

Need to know buffer conditions (any detergent?)

$75 per sample

Western Blot Assay

FOUR LANES ASSAYED

100ug antigen needed per lane

Protein concentration must be provided

Need to know buffer conditions (any detergent?)

Approximate molecular weight of protein of interest

First four lanes - $555

Each additional four lanes - $375

SDS Polyacrylamide Gel Electrophoresis of Antigens

Four lanes Assayed

Sample run under non-reducing conditions

Coomassie Blue Stained Gels

100ug antigen needed per lane

Protein concentration and approximate size must be provided

Need to know buffer conditions (any detergent?)First four lanes - $595

Each additional four lanes - $395

Effective 01/01/2024

ASSISTANCE IN PEPTIDE SEQUENCE SELECTION

PRF&L will select antigenic peptide sequences corresponding to the optimal antigenic domains using the protein sequence that you supply. After consultation with you regarding your project's goals, our expert staff will determine optional domains that are most likely to generate an antibody that will work best your specific application. Suggested animal host(s) will be determined. 

A detailed report will be provided.

$395 per protein analysis

CUSTOM PEPTIDE SYNTHESIS AND CONJUGATION

Package prices include at least 10mg for sequences up to 20 amino acids and 4mg conjugated to KLH. The peptide must contain a terminal cystine. If peptides do not contain terminal cystine, contact us for pricing. BSA and OVA are also available for conjugation at the same prices.

Peptides & Conjugation 80 percent purity 85 percent purity 90 percent purity 95 percent purity Over 95 percent purity

Up to 20 amino acids, 10 to 14mg of peptide $765 $880 $1015 $1195 By quotation

Additional Amino acid over 20, per amino aci $25 $26 $31 $38 By quotation

15 to 20mg of peptide + $4.70 per amino acid + $4.70 per amino acid + $4.70 per amino acid + $4.70 per amino acid By quotation

Modified peptides such as phospholoration are available. Please contact us for a quote.

KLH CONJUGATION FOR CUSTOMER SUPPLIED ANTIGENS

Up to 4mg of peptide conjugated to KLH - $460

Each additional mg conjugated to KLH - $67

Second carrier conjugation (BSA, OVA) at the same time – up to 4mg - $385

ELISA - SCREENING FOR TITER

Samples run in duplicate and actual titer reported. Recommended that minimum of two bleeds are tested; the desired sample along with either the preimmune or previous immune bleed.

$82 per sample

AFFINITY PURIFICATION

Antigens, either peptide or proteins, are linked to affinity matrices. Sera is then affinity purified. Multiple passes are run over the column. Price is based on volume of sera purified per batch.

  Initial Purification

Includes binding column Repeat Purification

using the same column

up to 25ml sera $580 $400

26 to 50ml sera $800 $645

51 to 100ml sera $1015 $850

Over 100ml sera By Quotation By Quotation

PROTEIN A OR PROTEIN G PURIFICATION

IgG, protein A or protein G will be purified from sera. Price is based on volume of sera purified per batch and run over host specific columns.

Volume per batch Price per batch

up to 5ml $235

6 to 25ml sera $445

26 to 50ml sera $810

51 to 100ml sera $1080

101 to 200ml $1540

Over 200ml By Quotation

PURIFICATION FROM EGG YOLKS

IgY will be purified from egg yolks by the polyethylene glycol precipitation method.

$385 for the first four egg yolks per batch

$30 for each additional egg yolk per batch when added to four yolk batch.

Affinity purification of IgY is available for an additional (four egg batch) - $600

Affinity purification - additional yolks - $125 each

TOTAL PROTEIN ASSAY

BCA or Bradford Method.

$225 per sample

SAMPLE DIALYSIS OR BUFFER EXCHANGE

$145 per sample up to 10ml

Shipping - Samples are generally shipped frozen using frozen ice packs via FedEx Priority Overnight Service within the United States. Dry ice is available for certian shipments as availabe. If you prefer that your samples to not be frozen, request that via the ordering Form and we will use wet ice packs instead. The price includes both the FedEx charge and packaging.

Zone 1 - $70

Zone 2 - $90

Zone 3 - $95

Zone 4 - $125

$35 packing fee to apply if using your shipping account

Outside the United States – By quotation & see below

Sera and Antibody Shipments Outside of the United States

Pocono Rabbit Farm & Laboratory is able to send antibody products many locations outside of the United States. We are in compliance with Regulation 1069/2009 and Regulation 142/2011 that enables us to ship to the European Union. We are listed on TRACES as PA-TEC-0009.

Pocono Rabbit Farm & Laboratory guarantees that we will send antibody products to locations outside the United States using our "best efforts" of our knowledge of the regulatory environment at the time of shipment.

Due to the increasing and dynamic nature of regulations, we will not make any guarantees for successful delivery of your antibody products. We strongly recommend that you advise us of your local customs regulations well in advance of shipment to avoid delays or additional charges.  We will pack shipments accordingly to expect delays.

Due to the required effort for international shipping, we will invoice you for permits, certificates, transportation charges, taxes, box charges, and an administrative fee. The administrative fee will vary from $80 to $500 depending on location, items shipped and time involved preparing documents.  We are not responsible for any taxes or duties that maybe applied (these charges will be passed on to recipient). 

Payment Terms For Services Provided By Pocono Rabbit Farm & Laboratory:

Generally, either a purchase order or a credit card number is required before starting any project, as requested on the Client Information Form.

All 91 Day, 70 Day, Mighty Quick, and Fusion Protein Protocols:

Credit Cards

PRF&L will charge the credit card for any pre-protocol services such as peptide synthesis, conjugation, or preimmune screening when the animals are first immunized.

PRF&L will charge the credit card again for the entire protocol when the first immune bleeds are shipped.

Optional Project extensions are charged periodically, generally at least every month for extended projects.

Concurrent and post protocol laboratory services are generally charged when the product is shipped or ELISA results are reported.

Both Mastercard and Visa are accepted. PRF&L does not accept American Express or Discover.

Purchase Orders

PRF&L will invoice any pre-protocol services such as peptide synthesis, conjugation, or preimmune screening when the animals are started, along with the animal protocol when the animals are first immunized.

Payment will be due before any immune sera is shipped.

Optional Project extensions are charged periodically, generally every month. Payment is net 30 days.

Concurrent and post protocol laboratory services are generally charged when the product is shipped or ELISA results are reported.

Custom Designed Protocols:

Cost and payment terms can be negotiated for large custom designed projects.

Laboratory services and other products when Antibody Production is not ordered

Credit card is required for all orders with value under $250.00. We will not accept a purchase order if the order value is under $250.00.

Generally, the credit card is charged or the invoice sent when the product is shipped.

Pocono Rabbit Farm and Laboratory expects prompt payment on all accounts.  Pocono Rabbit Farm & Laboratory reserves the right to suspend the protocol and or HOLD shipments if the account is more than 45 days past due. Interest rate of 15% annually will be chaged against past due accounts > then net 90 days. 

International Bank Checks and or ACH fees will be added to your invoices if you are going to pay by International Checks or by any wire transfrers.  Typically this is $20 -$30 per transaction.  We reccomend paying via a credit card or check drawn on a US Bank to avoid these fees.

We do offer ELISA services at $82 per sample