Considerations for using the ascites method include:

• Some hybridoma cell lines may not adapt well to in-vitro conditions, leading to negative or low yields.
• Monoclonal antibodies from mouse ascites fluids are crucial for experiments involving immune system components, autoimmune diseases, histocompatibility complexes, and more.
• Hybridoma cells may not replicate appropriate antibody activity in culture, particularly for certain protein antigens.
• Elution of monoclonal antibodies from purification columns can be sensitive, leading to denaturation or proteolysis and loss of antigen-binding activity.
• Serum-free cell culture methods may not produce sufficient quantities of monoclonal antibodies, especially anti-human isotypes crucial for vaccine evaluation.
• Monoclonal antibodies harvested from ascites fluids are essential for treatments such as removing tumor cells from bone marrow in neuroblastoma therapy.
• Culture methods may yield monoclonal antibodies with different glycosylation patterns, influencing antigen binding and biological functions.
• Contaminated myeloma cells producing monoclonal antibodies may need to be passaged through mice.
• Some cell lines that adapt to tissue culture conditions may lose production efficiency over time.