BEGINING A

NEW PROJECT

A woman is sitting at a table in a lab holding a test tube.

Step By Step

Are you ready to embark on a groundbreaking research project? Whether you're aiming to develop novel antibodies, synthesize custom peptides, or initiate hybridoma development, Pocono Rabbit Farm & Laboratory (PRF&L) is prepared to guide you through the process with expertise and efficiency. Before diving in, let's ensure we're on the same page:

  • Do you have antigen ready to go – or is some work needed first?
  • Do you need a peptide designed from your protein sequence?
  • Do you have a peptide sequence picked out?
  • Do you already have the peptide and need it to be conjugated?
  • Are you not sure?

Are you ready to embark on a groundbreaking research project? Whether you're aiming to develop novel antibodies, synthesize custom peptides, or initiate hybridoma development, Pocono Rabbit Farm & Laboratory (PRF&L) is prepared to guide you through the process with expertise and efficiency. Before diving in, let's ensure we're on the same page:

  • Do you have antigen ready to go – or is some work needed first
  • Do you need a peptide designed from your protein sequence
  • Do you have a peptide sequence picked out
  • Do you already have the peptide and need it to be conjugated
  • Are you not sure?

Starting a new project with Pocono Rabbit Farm & Laboratory (PRF&L) is a straightforward process designed to ensure clarity and efficiency at every step. Here's a step-by-step guide to get started:

  1. Complete Client Information Form: Obtain and fill out the Client Information Form. This form is essential for setting up your account before any services can begin.
Client Information Form

    2. Obtain Purchase Order: Choose one of our IACUC approved protocols, request a quote and complete the order form.

Polyclonal Antibidy Form

3. Prepare Antigen (if applicable): If you're sending antigen for antibody production, include the antigen along with the completed forms. Clearly label all tubes with antigen names and concentrations, and place them in a small bag or larger tube. Ship to:                                                                                                        Pocono Rabbit Farm & Lab
306 Dutch Hill Road
Canadensis, PA 18325

4. Include a cover letter detailing your shipment and related information.

5. Submit Peptide Design/Making Form (if applicable): If you're having PRF&L design and/or make your peptide, submit the Peptide Design/Making Form via email or fax.

6. Confirmation and Start Date: Once PRF&L receives the antigen/forms, they will review the materials and contact you with confirmation of receipt. They may ask any clarifying questions and provide you with a start date and information on when to expect the first shipment of serum.



By following these steps, you can initiate your project with PRF&L efficiently and ensure that all necessary information and materials are provided for seamless progress.

  • Do I Need Dry Ice When I Send My Antigen?

    Send the antigen at the conditions that you feel that the antigen will remain most stable. As a rule, most proteins should be shipped on Dry Ice. Peptides and conjugated peptides in solution can be sent on ice packs. Gel slices should also be sent on ice packs. Most lyophilized antigens are stable at room temperature and can be sent without any ice at all. Cells are usually sent with ice packs. Antigen bound to nitrocellulose membranes can be sent at room temperature.

  • How Much Dry Ice is Enough?

    Generally, three pounds of dry ice is plenty. Use a styrofoam container with walls at least 1 inch thick. Even if the package is delayed one day, three pounds is enough for the extra day of transit.

  • How Should I Send Gel Slices?

    We suggest putting the gel slice into a conical or eppendorf tube with a few drops of distilled water. The idea is to keep the gel moist in transit and in storage. Estimate how much gel there is in the tube. Send using a styrofoam box using a few ice packs to keep it cool. Please do not emulsify the gel, allow us to do it. Also, do not aliquot the gel as it is easier for us to work with it in one unit. Please do not freeze gels - sometimes they are difficult to work with after freezing.

  • What Carrier Should I Use?

    We suggest sending all perishable antigens overnight. We suggest using either FedEx or UPS as they come to our facility daily. We generally receive all packages by 4 PM Monday through Friday; our area does not have AM delivery. Please avoid using the USPS (United States Postal Service) for antigen shipments.

  • When Should I Send the Antigen?

    Please ship to us on Monday, Tuesday, or Wednesday, so that we can expect to receive on Tuesday, Wednesday, or Thursday. Please do not send perishable antigens on Thursdays. Even the most reliable carriers can have delays, so if the package that you send on Thursday is delayed one day, it will not come here until Monday.

  • Where Should I Send the Antigen?

    Shipping address is:

    Pocono Rabbit Farm & Lab

    306 Dutch Hill Road

    Canadensis, PA 18325

  • Can I Have My Antigen Come to You on a Saturday?

    No! All the overnight carriers will not deliver to our town on Saturdays.



  • How much antigen is needed for protocols?

    500ug per animal for every two animals we would need 1mg

  • What is the recommended purity for Peptide Synthesis?

    We recommend the minimum of 80% purity when making a peptide.

  • What is the recommended concentration?

    We like to recommend 1mg/ml for the concentration.

Ready to hop in?

GET A QUOTE TODAY!

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