close

click image to close
Home  >  Pricing

 

Monoclonal Antibody Production by In-Vivo Methods (Ascites Production)

Price per cell line, includes cell line preparation (when provided with Mycoplasma free live cell line) for immunization into 5 Balb/c Mice ( or another hybridoma specific strain maybe additional),  all necessary procedures and husbandry for a typical production run which is based on removing ascites fluid a maximum of 3 times per mouse followed by euthanasia and disposal. Yields differ greatly between cell lines but if the line is a reasonable producer, 3 to 10 ml per mouse can be anticipated.

$ 699.00


Each additional mouse added to production run by passing on the line from mouse to mouse. $99.00 ea

 

Protein G Purification of Ascites Fluid

(~ yield 0.5 - 2mg or >  / ml of neat ascites fluid)

25 ml or less .. $375

up to 50 ml    .. $500

up to 100 ml  .. $725

 

Custom Monoclonal Antibody Production

by In-Vitro Methods . . . . . . . . . . . . . . . . . . . . . . . . . . $1,450

Yield of approximately 50 - 200mg per batch The customer's cell line is amplified using three liter tissue culture polyethylene bags. Monoclonal antibodies are isolated using a protein G column. Available only for IgG producing lines.

 

Other protocols are available and the success with a certain protocol is

dependent on the cell line. Please consult with our technical staff prior

to starting an in vitro project.

 

Initial Cell Culture - When lines are supplied to us frozen.

We will culture, expand, and assess quality prior to ascites or in vitro

Production (ELISA method), per line . . .  . . . . . . . . . . . . .  $450

 

Amplification & Cryopreservation 10 vials @ 1 to 2 million cells

per vial per line and storage for the first year in liquid nitrogen . $450

Each additional year . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  $250

 

Mycoplasma Testing

First line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . $350

Each additional lines to be tested at the same time . . . . . . . . . .$150

 

Isotyping

First line / Antibody . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . $150

Each additional lines to be tested at the same time . . . . . . .  . . .  $75

 

 

 

Quantitation of Mouse IgG in hybridoma supernatant or ascites

First sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . $250

Each additional sample at the same time . . . . . . . . . . . . . . .   . . .$75

 

Conjugation to Biotin or other targets

Conjugation of up to 10mg of antibody to biotin or other target . . $450

Conjugation of same antigen to an additional carrier . . . . .           $250

 

AFFINITY PURIFICATION OF MONOCLONAL ANTIBODIES

Covalently link 1 - 2mg of antigen to column through amino groups.

$750 for 30ml sera or less

$1000 for 31-100ml sera

please inquire about higher volumes

 

ANTIBODY TITRATION BY ELISA

TITRATION ASSAY IN 96 WELL MICROPLATE ASSAY PERFORMED IN DUPLICATE

10 - 20ug antigen needed per plate for coating

Protein concentration must be provided

Need to know buffer conditions (any detergent?)

$95 for first sample

$55 for each additional sample to same antigen

 

 

WESTERN BLOT ASSAY

FOUR LANES ASSAYED

100ug antigen needed per lane

Protein concentration must be provided

Need to know buffer conditions (any detergent?)

Approximate molecular weight of protein of interest

$230 for first four lanes

$95 for each additional four lanes

 

 

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS

OF ANTIGENS

FOUR LANES ASSAYED

SAMPLE RUN UNDER REDUCING CONDITIONS

COOMASSIE BLUE STAINED GELS

100ug antigen needed per lane

Protein concentration and approximate size must be provided

Need to know buffer conditions (any detergent?)

$185 for first four lanes

$75 for each additional four lanes