Prices as of 9/01/2022

28 DAY MIGHTY QUICK PROTOCOL PACKAGE

All 28 Day Mighty Quick Protocols include setup, prebleed, three immunizations (including immune stimulators), one large immune bleed, final bleed, and maintenance. This protocol can be extended if additional sera is required. Price is per animal, unless noted, and the estimated volume of sera is shown.

49 DAY MIGHTY QUICK PROTOCOL PACKAGE

All 49 Day Mighty Quick Protocols include setup, prebleed, four immunizations (including immune stimulators), four large immune bleeds, final bleed, and maintenance. This protocol can be extended if additional sera is required. Price is per animal, unless noted, and the estimated volume of sera is shown.

70 DAY POLYCLONAL ANTIBODY PRODUCTION

70 Day Antibody Production Package includes setup, prebleed, four immunizations, one small immune bleed, one large immune bleed, and maintenance. This protocol can be extended if additional sera is required. This protocol is based on the PRF&L Traditional 70 Protocol offered in the past.

84 DAY FUSION PROTEIN ANTIGEN POLYCLONAL ANTIBODY PRODUCTION

84 Day Fusion Protein Protocol, a favorite for many years at Pocono Rabbit Farm & Laboratory. This protocol uses an IV injection at day 56 as a way to improve the antibody. This p365ackage includes setup, prebleed, six immunizations, two large immune bleeds, and maintenance. This protocol can be extended if additional sera is required.

91 DAY POLYCLONAL ANTIBODY PRODUCTION

91 Day Antibody Production Package includes setup, prebleed, five immunizations, one small immune bleed, four large immune bleeds, and maintenance. All immunizations are subcutaneous for this package. This protocol can be extended if additional sera is required.

PROJECT EXTENSIONS – ALL PRF&L PROTOCOLS CAN BE EXTENDED!

The purpose of illustrating the prices of the project extensions is to show what a typical project extension may cost. You are welcome to design your own extension. All procedures (boosts, bleeds, and maintenance) during the project extension phase of any project will be charged according to the Custom Protocol Antibody Production Price Schedule in effect at the time of the procedure.

Project Extension Prices shown below are for illustrative purposes only!

Actual charges will be calculated and billed monthly using the Custom Protocol Antibody Production Price Schedule in effect at the time of the procedure.

Custom Protocol Antibody Production Price Schedule

Project Extension A:

Boost and Bleed-Includes 1 boost immunization, 1 additional bleed, and 28 days additional maintenance

Project Extension B:

Production-Includes 4 production bleeds, 1 boost immunization, and 28 days additional maintenance

Project Extension C:

Hold-Animals are held pending your decision to proceed. No boosts or bleeds.  Prices below is the maintenance fee per day per animal.

FINAL BLEED/EXSANGUINATION - WITH CARCASS DISPOSAL

ADD ELISA SCREENING TO YOUR PROJECT

$75 per sample (more antigen may be required)
This price only available if scheduled at the start of the project.

PREIMMUNE SCREENING

Price includes the bleed and two weeks maintenance while you evaluate the bleeds.

Price Schedule for Monoclonal Antibody Production
Including Ascites Production

effective 09/01/2022

Monoclonal Antibody Production by In-Vivo Methods (Ascites Production) - inquire

Price per cell line, includes cell line preparation (when provided with Mycoplasma free live cell line) for immunization into 5 Balb/c Mice ( or another hybridoma specific strain maybe additional), all necessary procedures and husbandry for a typical production run which is based on removing ascites fluid a maximum of 3 times per mouse followed by euthanasia and disposal. Yields differ greatly between cell lines but if the line is a reasonable producer, 3 to 10 ml per mouse can be anticipated.

Each additional mouse added to production run by passing on the line from mouse to mouse - inquire

Protein G Purification of Ascites Fluid

approximate yield 0.5 to 2mg or > /ml of neat ascites fluid.
25 ml or less - $350
up to 50 ml - $755
up to 100 ml - $1025

25million cells per vial per line, including storage for the first year in liquid nitrogen - inquire

Each additional year of storage - inquire

Mycoplasma Testing

First line - inqiure
Each additional lines to be tested at the same time - inquire

Isotyping

First line / Antibody - inquire
Each additional line to be tested at the same time - inquire

Quantitation of Mouse IgG in hybridoma supernatant or ascites

First Sample - inquire
Each additional sample at the same time - inquire

Conjugation to Biotin or other targets (some targets may be more)

Conjugation of up to 10mg of antibody to biotin or other target - $595
Conjugation of same antigen to an additional carrier - $395

Antibody Titration by ELISA

TITRATION ASSAY IN 96 WELL MICROPLATE
ASSAY PERFORMED IN DUPLICATE
10 - 20ug antigen needed per plate for coating
Protein concentration must be provided.
Need to know buffer conditions (any detergent?)
$75 per sample

Western Blot Assay

FOUR LANES ASSAYED
100ug antigen needed per lane
Protein concentration must be provided
Need to know buffer conditions (any detergent?)
Approximate molecular weight of protein of interest
First four lanes - $555
Each additional four lanes - $375

SDS Polyacrylamide Gel Electrophoresis of Antigens

Four lanes Assayed
Sample run under non-reducing conditions
Coomassie Blue Stained Gels
100ug antigen needed per lane
Protein concentration and approximate size must be provided
Need to know buffer conditions (any detergent?)First four lanes - $595
Each additional four lanes - $395

Effective 09/01/2022

ASSISTANCE IN PEPTIDE SEQUENCE SELECTION
PRF&L will select antigenic peptide sequences corresponding to the optimal antigenic domains using the protein sequence that you supply. After consultation with you regarding your project's goals, our expert staff will determine optional domains that are most likely to generate an antibody that will work best your specific application. Suggested animal host(s) will be determined. 
A detailed report will be provided.
$395 per protein analysis

CUSTOM PEPTIDE SYNTHESIS AND CONJUGATION
Package prices include at least 10mg for sequences up to 20 amino acids and 4mg conjugated to KLH. The peptide must contain a terminal cystine. If peptides do not contain terminal cystine, contact us for pricing. BSA and OVA are also available for conjugation at the same prices.

Modified peptides such as phospholoration are available. Please contact us for a quote.

KLH CONJUGATION FOR CUSTOMER SUPPLIED ANTIGENS

Up to 4mg of peptide conjugated to KLH - $445
Each additional mg conjugated to KLH - $65
Second carrier conjugation (BSA, OVA) at the same time – up to 4mg - $370

ELISA - SCREENING FOR TITER
Samples run in duplicate and actual titer reported. Recommended that minimum of two bleeds are tested; the desired sample along with either the preimmune or previous immune bleed.

$75 per sample

AFFINITY PURIFICATION
Antigens, either peptide or proteins, are linked to affinity matrices. Sera is then affinity purified. Multiple passes are run over the column. Price is based on volume of sera purified per batch.

PROTEIN A OR PROTEIN G PURIFICATION
IgG, protein A or protein G will be purified from sera. Price is based on volume of sera purified per batch and run over host specific columns.

PURIFICATION FROM EGG YOLKS
IgY will be purified from egg yolks by the polyethylene glycol precipitation method.
$375 for the first four egg yolks per batch
$28 for each additional egg yolk per batch when added to four yolk batch.
Affinity purification of IgY is available for an additional (four egg batch) - $575
Affinity purification - additional yolks - $120 each

TOTAL PROTEIN ASSAY
BCA or Bradford Method.
$220 per sample

SAMPLE DIALYSIS OR BUFFER EXCHANGE
$135 per sample up to 10ml

Shipping - Samples are generally shipped frozen using frozen ice packs via FedEx Priority Overnight Service within the United States. Dry ice is available for certian shipments as availabe. If you prefer that your samples to not be frozen, request that via the ordering Form and we will use wet ice packs instead. The price includes both the FedEx charge and packaging.

Zone 1 - $59
Zone 2 - $78
Zone 3 - $89
Zone 4 - $98

Outside the United States – By quotation & see below

Sera and Antibody Shipments Outside of the United States

Pocono Rabbit Farm & Laboratory is able to send antibody products many locations outside of the United States. We are in compliance with Regulation 1069/2009 and Regulation 142/2011 that enables us to ship to the European Union. We are listed on TRACES as PA-TEC-0009.

Pocono Rabbit Farm & Laboratory guarantees that we will send antibody products to locations outside the United States using our "best efforts" of our knowledge of the regulatory environment at the time of shipment.

Due to the increasing and dynamic nature of regulations, we will not make any guarantees for successful delivery of your antibody products. We strongly recommend that you advise us of your local customs regulations well in advance of shipment to avoid delays or additional charges.  We will pack shipments accordingly to expect delays.

Due to the required effort for international shipping, we will invoice you for permits, certificates, transportation charges, taxes, box charges, and an administrative fee. The administrative fee will vary from $80 to $500 depending on location, items shipped and time involved preparing documents.  We are not responsible for any taxes or duties that maybe applied (these charges will be passed on to recipient). 

Payment Terms For Services Provided By Pocono Rabbit Farm & Laboratory:

Generally, either a purchase order or a credit card number is required before starting any project, as requested on the Client Information Form.

All 91 Day, 70 Day, Mighty Quick, and Fusion Protein Protocols:

Credit Cards

1. PRF&L will charge the credit card for any pre-protocol services such as peptide synthesis, conjugation, or preimmune screening when the animals are first immunized.
2. PRF&L will charge the credit card again for the entire protocol when the first immune bleeds are shipped.
3. Optional Project extensions are charged periodically, generally at least every month for extended projects.
4. Concurrent and post protocol laboratory services are generally charged when the product is shipped or ELISA results are reported.
5. Both Mastercard and Visa are accepted. PRF&L does not accept American Express or Discover.

Purchase Orders

1. PRF&L will invoice any pre-protocol services such as peptide synthesis, conjugation, or preimmune screening when the animals are started, along with the animal protocol when the animals are first immunized.
2. Payment will be due before any immune sera is shipped.
3. Optional Project extensions are charged periodically, generally every month. Payment is net 30 days.
4. Concurrent and post protocol laboratory services are generally charged when the product is shipped or ELISA results are reported.

Custom Designed Protocols:

Cost and payment terms can be negotiated for larg custom designed projects.

Laboratory services and other products when Antibody Production is not ordered

Credit card is required for all orders with value under $250.00. We will not accept a purchase order if the order value is under $250.00.

Generally, the credit card is charged or the invoice sent when the product is shipped.

Pocono Rabbit Farm and Laboratory expects prompt payment on all accounts.  Pocono Rabbit Farm & Laboratory reserves the right to suspend the protocol and or HOLD shipments if the account is more than 45 days past due. Interest rate of 15% annually will be chaged against past due accounts > then net 90 days. 

International Bank Checks and or ACH fees will be added to your invoices if you are going to pay by International Checks or by any wire transfrers.  Typically this is $20 -$30 per transaction.  We reccomend paying via a credit card or check drawn on a US Bank to avoid these fees.